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                "",
                "1) The microscope system must incorporate efficient and customizable computational clearing methods that makes it possible to readily distinguish real molecular signals from background signals from acquired wide-field images of thick tissue sections (> 100 \u03bcm), zebra fish embryos, spheroids and organoids. This type of samples typically has strong out-of-focus background signal that in many instances completely overwhelms the in-focus specific signal such that further analysis by this approach is not possible. While the standard experimental approach in these cases has been to image these more challenging samples on much more technologically demanding (and much more expensive) confocal microscopes, recent image processing approaches has shown that it is also possible to resolve such signals from images that have been acquired on a conventional wide-field microscope. For this purpose, it is essential that the incorporation of computational clearing methods enable a live-view format, such that image acquisition settings can be readily adjusted on the fly based on the resulting computationally cleared image, and also that it can be demonstrated that the linearity of the signal intensity is maintained in computationally cleared images. It is furthermore essential that the microscope system incorporates additional image restoration algorithms, so called image de-convolution, for further image enhancement of raw image data from the effects of diffraction. Incorporated de-convolution methods applied to sub-diffraction sized bead images should be able to demonstrate a 2X improvement in lateral resolution and 2.5X improvement in axial resolution. ",
                "",
                "2) The microscope must be capable of 5-colour high-specificity, high-speed, high-throughput, and high-resolution sequential wide-field fluorescence imaging of a broad range of biological specimen, ranging from single live cells to thick tissue sections, at high detection sensitivity. The proposed system configuration must furthermore include a motorized stage along with software control that makes it easy to acquire large tiled images as well multi-position experiments in an assortment of multi-well plate formats. ",
                "",
                "3) The microscope system must include a capability of imaging samples that have alternatively been stained by colorimetric histology stains such as haematoxylin and eosin.",
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                "4) The microscope system must include a black cage incubator with temperature and CO2 control to enable live cell imaging under physiological conditions. ",
                "",
                "According to the Act on Public Procurement and Concession Contracts (1397\/2016) section 40, when only a certain supplier can implement the procurement for a technical reason, provided also that there are no reasonable alternatives or substitute solutions, and that the absence of competition is not due to an artificial narrowing of the terms and conditions of the procurement, the contract may be awarded only to a particular supplier, Contracting authorities may award public contracts by a direct award. Leica Thunder Imager 3D Live Cell microscope, supplied by Immuno Diagnostica Oy, is the only instruments in the market that meet all the specific and necessary technical requirements to fulfil the uncompromising needs of the acquiring department. Prior to making decision the Contracting authority has inspected the relevant markets and available products and after careful consideration found its' conclusion grounded."
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